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Santa Cruz Biotechnology sox2
Sox2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1811 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sox2/Sox-2+Antibody/10__3390_slash_antiox15101254-72-17-21
Average 96 stars, based on 1811 article reviews
sox2 - by Bioz Stars, 2026-10
96/100 stars

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Related Articles

Incubation:

Article Title: PrP C Glycoprotein Modulates Atmospherically Relevant Artificial Particulate Matter-Induced Development of Lung Cancer in Mice.
Article Snippet: .. The membranes were blocked with 5% skim milk (Bio- Rad) and then incubated with specific primary antibodies against Kras (TA801672-Origene), CK7 (ET1609-63-Huabio), Sox2 (sc365823-Santa Cruz Biotechnology), Ki67 (ab16667-Abcam), TTF1 (sc-53136-Santa Cruz Biotechnology), c-Myc (sc-42 Santa Cruz Biotechnology), PrPC (A03202- SPI bio), HIF-1α (NB-100-449Novus Biologicals), COX2 (BS1076-BioWord), IL-1β (sc-52012Santa Cruz Biotechnology), TNF-α (AB1793-Abcam), Sirt1 (Millipore, Billerica, MA),p-p53 (sc-71786 Santa Cruz Biotechnology), p53 (sc-55476 Santa Cruz Biotechnology), Caspase-1 (2225 Cell Signaling), and γH2AX (AB26350 Abcam). β-actin served as an internal control. .. Signals were visualized by a chemiluminescent detection kit (Amersham Pharmacia Biotech) and analyzed by a densitometric scanner (FUSION SOLO 4S.

Article Title: On-demand mild photothermal cascade platform reprogramming mitochondrial immunity for tendon rejuvenation
Article Snippet: .. After washing, cells were incubated with primary antibodies against Ki67 (ab15580, Abcam), Phosphorylated Histone H2AX (γ-H2AX) (ab81299, Abcam), SOX2 (sc-365964, Santa Cruz), Type I Collagen (COL1) (ab138492, Abcam), tenomodulin (TNMD) (ab203676, Abcam; sc-51813, Santa Cruz), Scleraxis (SCX) (sc-518082, Santa Cruz), IRF3 (ab68481, Abcam), Transcription Factor p65/RELA (P65) (A22331, Abclonal), Cyclin-Dependent Kinase Inhibitor 2A (p16INK4a) (P16) (sc-1661, Santa Cruz), P53 (10442-1-AP, Proteintech), Inducible Nitric Oxide Synthase (iNOS) (ab178945, Abcam), Arginase-1(Arg-1) (ab96183, Abcam), HSP70 (sc-32239, Santa Cruz), IL-6 (ab233706, Abcam), Matrix Metalloproteinase 13 (MMP13) (ab39012, Abcam), Double-stranded DNA (dsDNA) Marker (sc-58749, Santa Cruz), and Translocase of Outer Mitochondrial Membrane 20 (TOMM20) (11802-1-AP, Proteintech). .. After incubation with fluorophore-conjugated secondary antibodies, Rhodamine-Phalloidin (C2203S, Beyotime, China), and DAPI (40728ES03, Yeasen, China), fluorescence images were captured with a confocal microscope and analyzed using ImageJ for fluorescence intensity and co-localization.

Article Title: The Effects of Neural Stem Cell-Derived Exosomes in the Improvement of Passive Avoidance Memory: A Behavioral, Molecular, and Electrophysiological Study in Adult Male Wistar Rats.
Article Snippet: 1 Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran 2 Department of Physiology, Faculty of Veterinary, Shahid Chamran University of Ahvaz, Ahvaz, Iran 3 Professor of Veterinary Pathology, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, South Australia, Australia Abstract Novel therapeutic approaches, such as exosome therapy, have garnered considerable attention for the treatment of central nervous system (CNS)-related disorders.. This study aimed to investigate the effect of Neural stem cell-derived exosomes(Exo-NSC) on improving behavioral, molecular, and electrophysiological symptoms.. Rats were divided into: control, lesioned groups (Alz, Alz + saline), treatments (Alz + NSC, Alz + Exo-NSC). the nucleus basalis of meynert (NBM) was lesioned using electrical lesion.

Article Title: PrP C Glycoprotein Modulates Atmospherically Relevant Artificial Particulate Matter-Induced Development of Lung Cancer in Mice.
Article Snippet: .. Tissue slides were incubated overnight at 4 °C with specific antibodies, including c-Myc (sc-42-Santa Cruz Biotechnology), Sox2 (sc-365823-Santa Cruz Biotechnology), TTF1 (sc-53136-Santa Cruz Biotechnology), K-ras (TA801672-Origene), Ki67 (ab16667-abcam), CK7 (ET1609-63 Huabio), PrPC (A03202-SPI bio), HIF-1α (NB100-449-Novus Biologicals), COX2 (BS1076-BioWord), IL-1β (sc52012-Santa Cruz Biotechnology), TNFα (AB1793-Abcam), 8- OHdG (Ab62623-Abcam), CD4 (25229-Cell Signaling), CD8 (sc7970-Santa Cruz Biotechnology) and VEGF-A (BS2853-Bio World). https://doi.org/10.1021/acs.est.5c08365 Environ. ..

Control:

Article Title: PrP C Glycoprotein Modulates Atmospherically Relevant Artificial Particulate Matter-Induced Development of Lung Cancer in Mice.
Article Snippet: .. The membranes were blocked with 5% skim milk (Bio- Rad) and then incubated with specific primary antibodies against Kras (TA801672-Origene), CK7 (ET1609-63-Huabio), Sox2 (sc365823-Santa Cruz Biotechnology), Ki67 (ab16667-Abcam), TTF1 (sc-53136-Santa Cruz Biotechnology), c-Myc (sc-42 Santa Cruz Biotechnology), PrPC (A03202- SPI bio), HIF-1α (NB-100-449Novus Biologicals), COX2 (BS1076-BioWord), IL-1β (sc-52012Santa Cruz Biotechnology), TNF-α (AB1793-Abcam), Sirt1 (Millipore, Billerica, MA),p-p53 (sc-71786 Santa Cruz Biotechnology), p53 (sc-55476 Santa Cruz Biotechnology), Caspase-1 (2225 Cell Signaling), and γH2AX (AB26350 Abcam). β-actin served as an internal control. .. Signals were visualized by a chemiluminescent detection kit (Amersham Pharmacia Biotech) and analyzed by a densitometric scanner (FUSION SOLO 4S.

other:

Article Title: Bioinspired Microgel Assembly of Liver-Derived Decellularized Extracellular Matrix Enhances Dentin-Pulp Regeneration via Stemness Awakening.
Article Snippet: Gradual loss of cellular potency during in vitro expansion is a primary challenge in the clinical translation of stem cell therapies.. This decline in regenerative potential significantly compromises regenerative endodontic therapy (RET)—an emerging strategy for reconstructing dentin-pulp complex.. Hence, we develop an injectable microgel scaffold composed of porcine liver-derived decellularized extracellular matrix (dECM) and gelatin methacryloyl (GelMA).

Blocking Assay:

Article Title: Spatial heterogeneity of lactylation: insights into gene expression, metabolism, and lactate transport in human embryonic stem cells
Article Snippet: PVDF membranes were stained with 0.1% ponceau S in 5% acetic acid before being washed with 1× Tris-buffered saline with Tween-20 (TBST) at room temperature before being blocked in 5% milk (#9999; Cell Signaling Technology) or 5% bovine serum albumin (BSA) (#ALB005.100; BioShop) in 1× TBST for 60 min at room temperature ( ). .. Following blocking, membranes were rewashed with 1× TBST at room temperature before incubating with the following primary antibodies: 1:1000 pan Kla (#1401; PTM BIO); 1:16,000 H3K18la (#1406; PTM BIO), 1:20,000 pan Kac (#9441S; Cell Signaling Technology), 1:200,000 Histone H3 (#ab1791; Abcam), 1:5000 CX43 (#C6219; Sigma-Aldrich); 1:1000 MCT1 (#ab90582; Abcam); 1:1000 LDHA (#2012; Cell Signaling Technology), 1:2000 OCT4 (#sc-5279; Santa Cruz Biotechnology); 1:2000 SOX2 (#sc-365823; Santa Cruz Biotechnology) ( ) overnight at 4°C. .. Following overnight incubation in the primary antibody, membranes were washed with 1× TBST at room temperature before incubation in the following secondary antibodies: goat anti-Rabbit IgG (H+L) HRP conjugate (#G-21234; Thermo Fisher Scientific) or goat anti-Mouse HRP conjugate (#170-5047; Bio-Rad) diluted in 5% milk (#9999S; Cell Signaling Technology) or 5% BSA (#ALB005.100; BioShop) in 1× TBST for 90 min at room temperature ( ).

Marker:

Article Title: On-demand mild photothermal cascade platform reprogramming mitochondrial immunity for tendon rejuvenation
Article Snippet: .. After washing, cells were incubated with primary antibodies against Ki67 (ab15580, Abcam), Phosphorylated Histone H2AX (γ-H2AX) (ab81299, Abcam), SOX2 (sc-365964, Santa Cruz), Type I Collagen (COL1) (ab138492, Abcam), tenomodulin (TNMD) (ab203676, Abcam; sc-51813, Santa Cruz), Scleraxis (SCX) (sc-518082, Santa Cruz), IRF3 (ab68481, Abcam), Transcription Factor p65/RELA (P65) (A22331, Abclonal), Cyclin-Dependent Kinase Inhibitor 2A (p16INK4a) (P16) (sc-1661, Santa Cruz), P53 (10442-1-AP, Proteintech), Inducible Nitric Oxide Synthase (iNOS) (ab178945, Abcam), Arginase-1(Arg-1) (ab96183, Abcam), HSP70 (sc-32239, Santa Cruz), IL-6 (ab233706, Abcam), Matrix Metalloproteinase 13 (MMP13) (ab39012, Abcam), Double-stranded DNA (dsDNA) Marker (sc-58749, Santa Cruz), and Translocase of Outer Mitochondrial Membrane 20 (TOMM20) (11802-1-AP, Proteintech). .. After incubation with fluorophore-conjugated secondary antibodies, Rhodamine-Phalloidin (C2203S, Beyotime, China), and DAPI (40728ES03, Yeasen, China), fluorescence images were captured with a confocal microscope and analyzed using ImageJ for fluorescence intensity and co-localization.

Membrane:

Article Title: On-demand mild photothermal cascade platform reprogramming mitochondrial immunity for tendon rejuvenation
Article Snippet: .. After washing, cells were incubated with primary antibodies against Ki67 (ab15580, Abcam), Phosphorylated Histone H2AX (γ-H2AX) (ab81299, Abcam), SOX2 (sc-365964, Santa Cruz), Type I Collagen (COL1) (ab138492, Abcam), tenomodulin (TNMD) (ab203676, Abcam; sc-51813, Santa Cruz), Scleraxis (SCX) (sc-518082, Santa Cruz), IRF3 (ab68481, Abcam), Transcription Factor p65/RELA (P65) (A22331, Abclonal), Cyclin-Dependent Kinase Inhibitor 2A (p16INK4a) (P16) (sc-1661, Santa Cruz), P53 (10442-1-AP, Proteintech), Inducible Nitric Oxide Synthase (iNOS) (ab178945, Abcam), Arginase-1(Arg-1) (ab96183, Abcam), HSP70 (sc-32239, Santa Cruz), IL-6 (ab233706, Abcam), Matrix Metalloproteinase 13 (MMP13) (ab39012, Abcam), Double-stranded DNA (dsDNA) Marker (sc-58749, Santa Cruz), and Translocase of Outer Mitochondrial Membrane 20 (TOMM20) (11802-1-AP, Proteintech). .. After incubation with fluorophore-conjugated secondary antibodies, Rhodamine-Phalloidin (C2203S, Beyotime, China), and DAPI (40728ES03, Yeasen, China), fluorescence images were captured with a confocal microscope and analyzed using ImageJ for fluorescence intensity and co-localization.

Immunofluorescence:

Article Title: Salidroside May Attenuate Palmitic Acid-Induced Bovine Oocyte Impairment Associated with Mitochondrial and Antioxidant Restoration
Article Snippet: .. The distribution of ICM and TE cells in blastocysts was assessed based on the immunofluorescence intensities of SOX2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-365823) and CDX2 (PeproTech, Cranbury, NJ, USA, 82659-1-RR), respectively. ..

Cell-Signaling:

Article Title: Transcriptomic Analysis of the Newborn Mammary Gland Identifies Prenatal Ethanol-Induced Remodeling of Insulin Signaling and Enhanced Proliferative Programming
Article Snippet: .. Proteins examined included IRS1(#3407), phospho-IRS1 (Ser612) (#3203), phospho-IRS1 (Tyr895) (#3070), phospho-Akt1 (Ser473) (#4060), ErbB3 (#12708), phospho-ERK1/2 (Thr202/Tyr204) (#9101) were purchased from Cell signaling Technology, EGFR (sc-03), IGF1R (sc-712), Akt1 (sc-5298), Erk2 (sc-1647), ERα (sc-8002), Sox2 (sc-398254), IGFBP5 (sc-515116) and β-Actin (sc-47778) were purchased from Santa Cruz Biotechnology. ..



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Phenotypic characterization of mouse NSPCs and the Pro-neurogenic effects of NPS loaded CEC/PEG-(CHO) 2 hydrogel scaffold In Vitro. (a) After 7 days in proliferation medium, isolated NSPCs formed neurospheres. Scale bar = 100 μm. (b–d) Neurospheres were positive for the NSPC markers Nestin, <t>SOX2,</t> and PAX6. Scale bar = 100 μm. (e–g) Quantification of Nestin + , SOX2 + , and PAX6 + cells. (h) Schematic diagram of NSPC isolation. (i) Schematic of the NSPC differentiation assay. (j) After 7 days of differentiation, immature neurons were immunostained for Tuj-1. Scale bar = 50 μm. (k) After 14 days, mature neurons were immunostained for MAP2. Scale bar = 50 μm. (l,m) Quantification of Tuj-1 + immature neurons (l) and MAP2 + mature neurons (m) . ** P < 0.01, *** P < 0.001. (n,o) qPCR analysis of <t>transcription</t> <t>factors</t> associated with neuronal differentiation at 7 (n) and 14 (o) days. * P < 0.05, ** P < 0.01, *** P < 0.001. (p) Schematic of the NSPC axon growth assay. (q) After 7 days in proliferation culture, axons were immunostained for GAP43. Scale bar = 200 μm. (r) Quantification of axon length. (s) qPCR analysis of transcription factors regulating axon growth. * P < 0.05, ** P < 0.01, *** P < 0.001.
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Phenotypic characterization of mouse NSPCs and the Pro-neurogenic effects of NPS loaded CEC/PEG-(CHO) 2 hydrogel scaffold In Vitro. (a) After 7 days in proliferation medium, isolated NSPCs formed neurospheres. Scale bar = 100 μm. (b–d) Neurospheres were positive for the NSPC markers Nestin, SOX2, and PAX6. Scale bar = 100 μm. (e–g) Quantification of Nestin + , SOX2 + , and PAX6 + cells. (h) Schematic diagram of NSPC isolation. (i) Schematic of the NSPC differentiation assay. (j) After 7 days of differentiation, immature neurons were immunostained for Tuj-1. Scale bar = 50 μm. (k) After 14 days, mature neurons were immunostained for MAP2. Scale bar = 50 μm. (l,m) Quantification of Tuj-1 + immature neurons (l) and MAP2 + mature neurons (m) . ** P < 0.01, *** P < 0.001. (n,o) qPCR analysis of transcription factors associated with neuronal differentiation at 7 (n) and 14 (o) days. * P < 0.05, ** P < 0.01, *** P < 0.001. (p) Schematic of the NSPC axon growth assay. (q) After 7 days in proliferation culture, axons were immunostained for GAP43. Scale bar = 200 μm. (r) Quantification of axon length. (s) qPCR analysis of transcription factors regulating axon growth. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Frontiers in Neuroscience

Article Title: A pH-responsive and self-healing CEC/PEG-(CHO) 2 hydrogel for controlled neuropeptide S delivery and endogenous neural repair after spinal cord injury

doi: 10.3389/fnins.2026.1933351

Figure Lengend Snippet: Phenotypic characterization of mouse NSPCs and the Pro-neurogenic effects of NPS loaded CEC/PEG-(CHO) 2 hydrogel scaffold In Vitro. (a) After 7 days in proliferation medium, isolated NSPCs formed neurospheres. Scale bar = 100 μm. (b–d) Neurospheres were positive for the NSPC markers Nestin, SOX2, and PAX6. Scale bar = 100 μm. (e–g) Quantification of Nestin + , SOX2 + , and PAX6 + cells. (h) Schematic diagram of NSPC isolation. (i) Schematic of the NSPC differentiation assay. (j) After 7 days of differentiation, immature neurons were immunostained for Tuj-1. Scale bar = 50 μm. (k) After 14 days, mature neurons were immunostained for MAP2. Scale bar = 50 μm. (l,m) Quantification of Tuj-1 + immature neurons (l) and MAP2 + mature neurons (m) . ** P < 0.01, *** P < 0.001. (n,o) qPCR analysis of transcription factors associated with neuronal differentiation at 7 (n) and 14 (o) days. * P < 0.05, ** P < 0.01, *** P < 0.001. (p) Schematic of the NSPC axon growth assay. (q) After 7 days in proliferation culture, axons were immunostained for GAP43. Scale bar = 200 μm. (r) Quantification of axon length. (s) qPCR analysis of transcription factors regulating axon growth. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The following primary antibodies were used: Nestin (rabbit, Proteintech, 1:400; NSPCs), SOX2 (rabbit, Proteintech, 1:400; NSPCs), PAX6 (rabbit, Proteintech, 1:400; NSPCs), TUJ1 (mouse, Proteintech, 1:400; immature neurons), MAP2 (mouse, Abcam, 1:400; mature neurons), GFAP (rat, Thermo Fisher, 1:400; astrocytes), GAP43 (rabbit, Proteintech, 1:400; axons), MMP-9 (rabbit, Abclonal, 1:400; secreted MMP-9), TNF-α (mouse, Proteintech, 1:400; pro-inflammatory cytokines), IL-10 (mouse, Proteintech, 1:400; anti-inflammatory cytokines), CD68 (rat, Abcam, 1:400; activated immune cells), CSPGs (mouse, Cell Signaling Technology, 1:400; glial scar), synaptophysin (SYP, rabbit, Proteintech, 1:400; presynaptic membranes), and cAMP (rabbit, Proteintech, 1:400; intracellular second messenger).

Techniques: In Vitro, Isolation, Differentiation Assay, Analysis, Growth Assay

Reagents details.

Journal: Stem cell research

Article Title: Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients with TTN mutations

doi: 10.1016/j.scr.2026.104065

Figure Lengend Snippet: Reagents details.

Article Snippet: Undifferentiated hPSC state marker , Mouse IgG1κ Anti-Sox2 , 1:200 , Santa Cruz Biotechnology Cat #sc-365823 , AB_10842165.

Techniques: Immunocytochemistry, Marker